(Download v262) for ruby-gsl 1.9 and 1.8
(Download v262p1) for ruby-gsl 1.10 and later
An implementation of helical image processing based on object-oriented scripting language Ruby.
Very detailed documentation including installation, commands, and programming interface.
User’s Guide (1.7MB pdf)written by Andrew Bodey when he studied mitotic kinesin motor at Carolyn Moores’ lab
This is a practical guide to use Ruby-Helix for helical reconstruction.
Special thanks to:
Masahiro Tanaka, who made NArray Ruby-Helix heavily depends on this library.
When you use Ruby-Helix or Unbend, please refer to the following publications:
Metlagel, Z., Y. S. Kikkawa, and M. Kikkawa
Ruby-Helix : An implementation of helical image processing based on object-oriented scripting language
J. Struc. Biol. 157:95-105, 2007
A new theory and algorithm for reconstructing helical structures with a seam.
Journal of Molecular Biology, 343:943-955, 2004
List of publications in which Ruby-Helix was used:
Kikkawa M, Hirokawa N.
High-resolution cryo-EM maps show the nucleotide binding pocket of KIF1A in open and closed conformations.
EMBO J. 2006 Sep 20;25(18):4187-94.
Mizuno N, Narita A, Kon T, Sutoh K, Kikkawa M.
Three-dimensional structure of cytoplasmic dynein bound to microtubules.
Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20832-7. Epub 2007 Dec 19.
Oda T, Hirokawa N, Kikkawa M.
Three-dimensional structures of the flagellar dynein-microtubule complex by cryoelectron microscopy.
J Cell Biol. 2007 Apr 23;177(2):243-52. Epub 2007 Apr 16.
Bodey AJ, Kikkawa M, Moores CA.
9-Angström structure of a microtubule-bound mitotic motor.
J Mol Biol. 2009 May 1;388(2):218-24
Peters C, Brejc K, Belmont L, Bodey AJ, Lee Y, Yu M, Guo J, Sakowicz R, Hartman J, Moores CA.
Insight into the molecular mechanism of the multitasking kinesin-8 motor.
EMBO J. 2010 Oct 20;29(20):3437-47